Fig. 4.

Lipid composition of mesenchymal stem cells. (A) Identified lipid classes by direct infusion high resolution MS and MS/MS experiments. In total, 360 lipids were identified. (B) Sum of double bonds in fatty acids for all major phospholipid classes. (C) Total length analysis of glycerophospholipids, as sum of fatty acids, pointing out the most abundant species per class. (D) Investigation at the fatty acid scan species level applying MS/MS experiments elucidates the unsaturation level and chain length per fatty acid and is demonstrating that this distribution is distinct between different glycerophospholipid classes. Examples of phosphatidylserine (PS) and phosphatidylinositol (PI) are given in (D), the upper panel displays the abundance of double bonds and fatty acid chain length (number of carbons), and the lower panel the number of total identified fatty acids with a certain number of double bonds and chain length (n = 3 technical replicates for all performed experiments).