Table 2.
IC50 values for ROP19 binding to MHC class II molecules obtained using the immune epitope databasea.
MHC II Alleleb | Start-Stopc
|
Percentile Rankd
|
||
---|---|---|---|---|
SAG1 | ROP19 | SAG1 | ROP19 | |
HLA-DRB1*01:01 | 12–26 | 5–19 | 0.88 | 0.04 |
35–49 | 160–174 | 2.74 | 0.04 | |
H2-IAb | 26–40 | 44–58 | 2.15 | 0.63 |
297–313 | 536–550 | 2.81 | 2.19 | |
H2-IAd | 21–35 | 374–388 | 0.34 | 0.67 |
168–182 | 539–553 | 1.22 | 0.18 | |
H2-IEd | 14–28 | 110–124 | 18.45 | 13.17 |
34–48 | 520–534 | 30.62 | 13.22 |
The immune epitope database (http://tools.immuneepitope.org/mhcii). The prediction was run three times.
H2-IAb, H2-IAd, and H2-IEd alleles are mouse MHC class II molecules; the HLA-DRB1*01:01 allele is a human MHC class II molecule.
We chose 15 amino acids for analysis each time.
Low percentile rank = high level binding, high percentile rank = low level binding, IC50 values = percentile rank.