Table 2. Summary of error rates.

Summary of the error rates and number of observed OTUs for each region.

Region	Error rate following (%)			Reads remaining (%)	Average no. of OTUsa
	Basic	Predicted	Precluster		Mockb	Mockc	Soil	Mouse	Human
V4	0.77	0.35	0.158	62.58	33.4	48.9	629.4	212.6	116.9
V1–V3	0.56	0.27	0.068	67.01	20.5	27.7	726.0	107.3	106.4
V3–V5	0.74	0.22	0.035	63.15	21.1	31.2	NA	112.3	83.8
V1–V5	0.57	0.21	0.019	59.29	19.8	27.2	694.4	65.9	84.9
V1–V6	0.60	0.21	0.031	59.90	20.0	46.5	693.0	75.8	89.4
V1–V9	0.68	0.21	0.027	51.33	19.5	40.4	NA	102.5	117.3

Notes.

^aThe number of OTUs is based on rarefaction of each sample to 1,000 sequences per sample; cells labeled ND reflect samples that did not have at least 1,000 sequences.

^bNumber of OTUs in the mock community when all chimeras were removed; in the absence of chimeras and sequencing errors, there should be 19 OTUs for all three regions.

^cNumber of OTUs in the mock community when chimeras were removed using UCHIME.