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Lane 1: full-length S. pombe α-actinin; lane 2: ABD; lane 3: ROD; lane 4: ABD-ROD; lane 5: ROD-EF; lane 6: EF-his; lane M: molecular weight markers: 250, 150, 100, 75, 50, 37, 25, 20, 15 and 10 kDa.
