Figure 4. Gel filtration of expressed S. pombe peptides.

The apparent molecular size of ABD (brown), ABD-ROD (blue), ROD (gold), ROD-EF (magenta) and EF (red) as well as of full-length α-actinin (black) were determinate by gel filtration on a Superdex™ 200 10/300GL, equilibrated with 50 mM Tris–HCl, pH 7.6, 200 mM KCl. Elution patterns were determined by absorbance at 280 nm. Inset. Stokes radii were determined using thyroglobulin (669 kDa, 8.58 nm), ferritin (440 kDa, 6.10 nm), aldolase (158 kDa, 4.81 nm), γ-globulin (158 kDa, 5.22 nm), bovine serum albumin (67kDa, 3.55 nm), ovalbumin (44 kDa, 2.80 nm), chymotrypsinogen (25 kDa, 2.09 nm) and myoglobulin (17 kDa, 1.90 nm) as molecular size references.