Table 2. Ranking and statistical analyses of candidate reference genes using BestKeeper, geNorm and NormFinder.
| BestKeeper | geNorm | NormFinder | |||||
|---|---|---|---|---|---|---|---|
| Ranking | r | Ranking | SD | Ranking | M | Ranking | SV |
| rpl8 | 0.908 | tbp | 0.294 | ppia2 | 0.111 | rpl8 | 0.137 |
| tbp | 0.863 | rpl8 | 0.343 | rpl8 | 0.125 | ppia2 | 0.154 |
| ppia2 | 0.862 | ppia2 | 0.350 | tbp | 0.133 | tbp | 0.157 |
| actb1 | 0.687 | actb1 | 0.396 | actb1 | 0.26 | actb1 | 0.287 |
| efl1a | 0.331 | efl1a | 1.358 | efl1a | 0.739 | efl1a | 1.128 |
| tuba1 | 0.201 | gapdh | 1.690 | gapdh | 1.084 | gapdh | 1.382 |
| gapdh | 0.148 | tuba1 | 2.773 | tuba1 | 1.482 | tuba1 | 2.088 |
Notes.
Abbreviations
- SD
- Standard deviation
- r
- Pearson product-moment correlation coefficient
- SV
- Stability value
- M
- M value of stability