Figure 4.

Transcriptional signatures accurately discriminate between symptomatic and asymptomatic rhinovirus (RV) detection. Unsupervised hierarchical clustering (euclidean distance, average linkage) of samples included in the training set (n = 44), the test set (n = 44), validation set A (n = 26), and an additional cohort of 14 RV+ asymptomatic children (total N = 125) grouped the samples in two main clusters (separated by dashed vertical line). The first cluster included the majority of RV− healthy control subjects (27 [93%] of 29) and all RV+ asymptomatic subjects (14 [100%] of 14), and the second cluster comprised the majority of RV+ inpatients (64 [98%] of 65) and RV+ outpatients (15 [88%] of 17). The optimal number and the stability of the clusters were determined by calculating average silhouette indices (Figure E3).