FIGURE 5.

IgdE is highly specific for porcine IgG. A, 2% porcine plasma was incubated with (+) or without (−) purified 10 nm rIgdE for 16 h at 37 °C. The reaction was analyzed by anti-porcine IgG, IgM, and IgA Western blots under reducing conditions. Only cleavage of IgG was observed (*). B, 0.5 mg/ml of IgG of different species was incubated with (+) or without (−) 10 nm purified rIgdE for 16 h at 37 °C and analyzed SDS-PAGE under reducing conditions. No cleavage of IgG derived from any other species than pig (*) was observed. The weak protein band of 37 kDa is a contaminant present in lysate preparation (see second lane) and not related to IgdE activity. Images of different gels have been assembled into one figure.