FIGURE 7.
IgdE is necessary for IgG cleavage by S. suis and IgG cleavage is independent of IdeSsuis. Concentrated (×10) supernatants of S. suis strains 10, 10ΔigdE, 10ΔideSsuis, and 10ΔideSsuis ΔigdE were incubated with 1% porcine plasma for 16 h at 37 °C. The reaction was analyzed by anti-IgG Western blot (A) or anti-IgM Western blot (B) under reducing conditions. IgG degradation (*) was only observed when incubated with supernatants of strains 10 and 10ΔideSsuis, meanwhile IgM degradation (*) only was observed upon incubation with supernatants of strains 10 and 10ΔigdE.