FIGURE 6.

Hippo signaling regulates Yki localization by impairing the binding between Yki and Importin α1. A, 14-3-3 cannot interact with YkiN, but can interact with YkiΔN. B, Hpo but not 14-3-3 affects the binding between Yki and Importin α1. S2 cells expressing the indicated constructs were immunoprecipitated and probed with the indicated antibodies. C, cellular localization of Myc-Yki but not that of Myc-Yki168A is influenced by Hpo. Co-immunoprecipitation experiment was performed, followed by Western blot analysis. D, function of Hpo depends on its kinase activity. S2 cells were transfected with the indicated constructs. The cell lysates were immunoprecipitated, followed by Western blot. E–H) Hpo inhibits Importin α1 induced Yki localization. S2 cells were immunostained with the indicated antibodies. I, cells in E–H were categorized based on the anti-Myc immunostaining pattern. 100 cells were counted in each case. J, interaction between Yki and Importin α1 was Ran-GTP sensitive. S2 cells were transfected with the indicated constructs. The cell lysates were immunoprecipitated, followed by Western blot analysis.