TABLE 1.

Specific activity and kinetic parameters of FadD32 orthologs

Protein	MtFadD32	MmFadD32	MsFadD32	CgFadD32
Identity (%)	100	92	74	39
Specific activity (nmol·min−1·mg−1)	5.37 ± 0.16	3.85 ± 0.12	39.91 ± 0.73	0.46 ± 0.03
Kinetic parameters
ATP
Km (μm)	248 ± 27	902 ± 114	112 ± 12
Vmax (μm·min−1)	0.14 ± 0.01	0.29 ± 0.01	0.17 ± 0.01
kcat (min−1)	0.35 ± 0.01	0.72 ± 0.03	4.33 ± 0.01
kcat/Km (min−1·μm−1)	0.0014 ± 0.0003	0.0008 ± 0.0003	0.0387 ± 0.0063
Lauric acid (C12)
Km (μm)	72.09 ± 5.75	103.2 ± 13.94	25.9 ± 1.6a
Vmax (μm.min−1)	0.82 ± 0.03	0.60 ± 0.39	0.45 ± 0.01
kcat (min−1)	2.04 ± 0.07	1.51 ± 0.09	11.29 ± 0.22
kcat/Km (min−1·μm−1)	0.028 ± 0.012	0.015 ± 0.007	0.438 ± 0.139
Myristic acid (C14)
Km (μm)	4.77 ± 0.65	5.76 ± 0.56	5.2 ± 0.5a
Vmax (μm.min−1)	0.39 ± 0.01	0.27 ± 0.01	0.17 ± 0.01
kcat (min−1)	0.98 ± 0.03	0.69 ± 0.01	4.18 ± 0.09
kcat/Km (min−1·μm−1)	0.206 ± 0.042	0.119 ± 0.025	0.800 ± 0.184

^a Data are from Ref. 17.