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. 2016 Feb 8;291(15):8048–8058. doi: 10.1074/jbc.M115.685313

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FIGURE 2. — POSs are mono-phosphorylated. Intact POSs (17 pmol as Man₂GlcNAc₂-P) prepared from glucose-deprived wild-type MEFs were analyzed by LC-ESI-MS. A, MS chromatograms of Hex_0–5HexNAc₂-P. Parentheses indicate magnification ratio. B, MS spectra of HexNAc₂-P (m/z 503.1285), Hex₁HexNAc₂-P (m/z 665.1806), Hex₂HexNAc₂-P (m/z 827.2333), Hex₃HexNAc₂-P (m/z 989.2844), Hex₄HexNAc₂-P (m/z 1151.3464), and Hex₅HexNAc₂-P (m/z 1313.3931). The average mass error for all detected POSs was 0.38 ppm, except that the mass error for Hex₅HexNAc₂-P was 5.82 ppm. Typical mass accuracy in external calibration was less than 5 ppm.