TABLE 1.
Strains, plasmids, and phages
| Description | Source/Reference | |
|---|---|---|
| Strains | ||
| GJ3161 | MC4100 galEp3 | Ref. 49 |
| MG1655 | ||
| GJ5147 (RS734) | MC4100 galEp3, Plac H-19B nutR-tR1- lacZYA | J. Gowrishankar |
| RS1019 | MC4100 galEp3, λRS45 lysogen carrying Plac-−λnutR-λtR1 −lacZYA | Ref. 22 |
| RS1038 | MC4100 galEp3, λRS45 lysogen carrying Plac- trpt′-lacZYA | Ref. 22 |
| RS1428 | MC4100 galEp3, λRS45 lysogen carrying PRM −racR/tRAC- lacZYA | Ref. 21 |
| RS1471 | MC4100 galEp3, λRS45 lysogen carrying PLac −AT-H19B nutR-tR1- lacZYA | |
| XL1-Red | endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac mutD5 mutS mutT Tn10 (TetR) | Stratagene |
| Phage | ||
| λRS45 | J. Gowrishankar | |
| Plasmids | ||
| pHyd3011 | ||
| pRS22 | pTL61 T with pT7A1-H-19B nutR-TR′T1T2-lacZYA, AmpR | Ref. 50 |
| pRS106 | pT7A1-trp t′-lacZ, AmpR | Ref. 43 |
| pRS431 | pTL61T with Plac − lacZYA by deletion of H-19B nutR-tR1 between HindIII and BamHI sites from pK8628, AmpR | Ref. 20 |
| pRS604 | T7A1-λTR1 fragment cloned at HindIII site of pRS22. | Ref. 41 |
| pRS649 | pCL1920 with rho (WT) with its own promoter cloned at HindIII and SacI sites. | Ref. 22 |
| pRS703 | nusA (WT-FL) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | Ref. 25 |
| pRS1165 | nusA (R258C-FL) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1251 | nusA (R258C) with nusA promoter cloned at BamHI/SalI sites of pCL1920 vector, SpecR, StrepR | This study |
| pRS1252 | nusA (G181D) with nusA promoter cloned at BamHI/SalI sites of pCL1920 vector, SpecR, StrepR | This study |
| pRS1396 | nusA (WT-SKK) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1447 | pTL61T with PLac- rrnG AT-H-19B nutR-tR1 -lac ZYA, AmpR | This study |
| pRS1472 | nusA (WT) with nusA promoter cloned at BamHI/SalI sites of pCL1920 vector, SpecR, StrepR | This study |
| pRS1520 | nusA (G181D-SKK) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1521 | nusA (R258C-SKK) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1522 | nusA (G181D-FL) cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1530 | pTL61T with PT7A1− rrnG AT-H-19B nutR-tR1 -lac ZYA, AmpR | This study |
| pRS1609 | pT7A1-AT-trp t′-lacZ, AmpR | This study |
| pRS1695 | nusA (WT-SKK) His-tagged, cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1696 | nusA (G181D-SKK) His-tagged, cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |
| pRS1697 | nusA (R258C-SKK) His-tagged, cloned at NdeI/SalI sites of pHYD3011 vector, AmpR | This study |