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. 2016 Feb 17;291(15):8150–8161. doi: 10.1074/jbc.M115.648717

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Dose-dependent binding activities of DGKη-PH to PI(3,4)P₂, PI(4,5)P₂, PI(3,4,5)P₂, PA, PC, and PS. Indicated amounts (1.6, 3.1, 6.3, 13, 25, 50, and 100 pmol) of PI(3,4)P₂, PI(4,5)P₂, PI(3,4,5)P₃, PA, PC, and PS were spotted onto a nitrocellulose membrane. The membrane was incubated with GST-tagged DGKη-PH. Lipid-bound DGKη-PH was detected by immunostaining with anti-GST antibody. A representative result of three independent experiments is shown. It was confirmed that lipid-bound GST alone was not detectable in this experiment (data not shown).