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Biosynthetic pathway of 24-methyl and 24-ethyl sterols in higher plants. This study determined that ArDWF1 catalyzes the direct reduction of a Δ24(28) sterol (red arrow) as opposed to the typical two-step mechanism that includes double-bond migration. To simplify the discussion, all structures are depicted as having 3β-hydroxy-Δ5 nuclei. However, this does not necessarily mean that the reactions proceed as the nuclei structure.
