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. 2016 Feb 17;291(15):8199–8213. doi: 10.1074/jbc.M115.674531

FIGURE 2.

FIGURE 2.

Overexpression of GSK3β in neural progenitors of GFAP/GSK3β. A–C, double immunofluorescence for GFAP (A, green channel) and β-galactosidase (B, red channel). C, merge panel with granule neurons in DG counterstained with DAPI (blue channel). The white arrow shows a double positive NPC located in the SGZ. The white arrowhead shows a double positive astrocyte in the hilus. D and E, orthogonal view of double positive NPC, which overexpress GSK3β. F–I, triple immunolabeling for GFAP (F, green), β-galactosidase (G, red), and S100β (H, gray). I, merge panel with DAPI staining in blue. The white arrows show a NPCs located in the SGZ, positive for β-galactosidase and negative for S100β. The white arrowhead shows a double positive non-neurogenic astrocyte. The thin white arrows show a mature astrocytes in the hilus and in the DG, which do not express the transgene. J and K, orthogonal view of double positive NPC, which overexpress GSK3β. L–N, double immunolabeling for BLBP (L, green) and β-galactosidase (M, red) in hippocampus of GFAP/GSK3β. N, merge panel with DAPI staining in blue. O and P, orthogonal view of double positive NPC, which overexpress GSK3β. Q–S, double immunolabeling for Sox2 (Q, green) and β-galactosidase (R, red) in hippocampus of GFAP/GSK3β. S, merge panel with DAPI staining in blue. T and U, orthogonal view of double positive NPC, which overexpress GSK3β.