FIGURE 15.

C3bBb(Mg²⁺) C3 convertase and C5b formation by using normal human serum detected by microplate/WB assays. A, C3bBb(Mg²⁺) complexes were obtained by incubating C3b-coated wells with 20% NHS at 37 °C for 30 min, in the presence 5 mm MgCl₂. C3bBb and C5b formation was evaluated as the presence of Bb (60 kDa) and α′-chain C5b (104 kDa) bands, respectively. FH could be visualized in the WB as a 150-kDa band. Results of a representative microplate/WB experiment of n = 3 are shown. B, schematic describing C3 and C5 convertase formation and C5b deposition on C3b-coated wells with 20% NHS.