FIGURE 7.

Effect of FH on C3bBb(Mg²⁺) C3 convertase formation and decay in the presence of P analyzed by microplate/WB assays. C3bBb(Mg²⁺) complexes were generated by incubating at 25 °C for 10 min C3b-coated wells with 1000 ng/ml FB, 5 ng/ml FD, and 10 mm MgCl₂ in the presence or in the absence of 114.5 ng/ml P and 2640 ng/ml FH. Spontaneous or FH-mediated decay was evaluated by further incubation at 25 °C for 10 min with buffer alone or added with 2640 ng/ml FH. The amount of C3bBb formed was calculated as the intensity of the Bb (60 kDa) band and reported in the bottom graphs as pixel²·10⁶. Results of a representative microplate/WB experiment of n = 3 are shown.