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. Author manuscript; available in PMC: 2017 Mar 2.
Published in final edited form as: J Am Chem Soc. 2016 Feb 19;138(8):2571–2575. doi: 10.1021/jacs.5b09764

Figure 1.

Figure 1

Ratiometric imaging with a dual versus single fluorophore biosensor. (a) The affinity reagent (AR) binds to the protein of interest (POI) only when the POI is in the active state. The changing intensity of the dye (red) relative to the fixed intensity of the fluorescent protein (FP) reflects POI binding. (b) POI activation is reflected simply in the ratio of emission at two different mero199 excitation wavelengths.