Skip to main content
. Author manuscript; available in PMC: 2017 Mar 2.
Published in final edited form as: J Am Chem Soc. 2016 Feb 19;138(8):2571–2575. doi: 10.1021/jacs.5b09764

Figure 3.

Figure 3

Migrating MEF cells microinjected with CRIB199, the biosensor based on mero199 conjugated to the CRIB domain, show Cdc42 activity in protrusions at the leading edge. Cells containing a mutant biosensor with reduced affinity for Cdc42 (mutantCRIB199) showed substantially less Cdc42 activation (images are scaled identically to show this). The pseudocolor scale shows the increase in ratio relative to the lowest 5% of ratio values in the cell (see Figure S5). These low values were similar for the two biosensors. The white arrow indicates the direction of movement.