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Fluctuation Theorem Applied to Barnase
| ΔG (kBT) | (kBT) | (kBT) | (kBT) | ΔG0 (kBT) |
|---|---|---|---|---|
| 1048 ± 5 | 40.0 ± 0.5 | 80 ± 3 | 915 | 14 ± 5 |
The free energy ΔG is obtained from the application of Jarzynski equality and corrected by an estimation of the bias (Eqs. S16 and S17, respectively). Once the different energetic contributions from the experimental setup are removed (Eq. S21 and Section S9), the free energy of formation of barnase is obtained.
