Figure 3.

BT-1 TALEN design and target diagram. A, The mannopine synthase (MAS) promoter drives the expression of the TALEN monomers. The pea (Pisum sativum) rbcT RBCSE9 acts as a gene terminator. A V5 epitope tag and an SV40 nuclear localization signal (NLS) reside on the N terminus. B, BFP target region schematic. The BT-1 TALE left and right binding domains are underlined. The site of BFP-to-GFP conversion (C→T) is in blue. BT-1 activity produces a DSB that can be repaired by NHEJ or through ssODNs, resulting in indels or BFP-to-GFP precision editing, respectively. Red bases are silent substitutions used to discourage BT-1 activity after conversion.