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. 2016 Feb 10;170(4):1917–1928. doi: 10.1104/pp.15.01696

Figure 4.

Figure 4.

Addition of ssODNs enhances genome editing in Arabidopsis protoplasts treated with TALEN BT-1. A, Imprecise NHEJ repair events in Arabidopsis protoplasts treated with BT-1 TALEN. B, Percentage of total indels as a result of BT-1 activity by length. C, Addition of ssODN to BT-1 TALEN-treated protoplasts significantly enhances the frequency of BFP-to-GFP conversion in Arabidopsis. Arabidopsis protoplasts were treated with BFP/41, BFP/101, or BFP/201 with and without BT-1 TALEN or BT-1 TALEN without ssODN. BFP-to-GFP edits were evaluated by cytometry 72 h after delivery. Data represent means ± se (n = 3).