Figure 9.

Targeted EPSPS edits provide herbicide tolerance to flax calli and regenerated plants. A, Flax wild-type (Wt) calli and calli derived from event A23, which contains T178I and P182A edits in EPSPS gene 2, were cultured in six-well dishes on medium containing a range of glyphosate concentrations. Images were captured 14 d after the initiation of treatment. Each image corresponds to one well. B, Mean fresh weight per well of wild-type and A23 calli treated with glyphosate after 21 d. Data represent means ± se (n = 3). C, Greenhouse-hardened wild-type and A23 whole plants in soil were treated with 10.5 or 21 mm glyphosate or surfactant only by spray application. Images were captured 6 d after glyphosate application. This experiment was repeated multiple times with similar results. Bar = 2 cm.