Table I. Summary of flax CRISPR/Cas9 experiments targeting EPSPS.

Experiment	Deep Sequencing of Microcoloniesa		Calli Genotyping Resultsb
	Precise Editsc	Indels	Calli Screened	Calli with Precise Edits
FC-1	0.23%	19.8	5,167	8 (0.15%)
FC-2	0.10%	19.2	4,601	4 (0.08%)
FC-3	0.09%	19.6	NS

NS, Experiment was not screened.

^aGenomic DNA was isolated from pools of approximately 10,000 microcolonies and then used as a template to amplify the target region.

^bIndividual callus was screened first by allele-specific PCR and then confirmed by Sanger sequencing.

^cSequences with T97I (ACA→ATA) and P101A (CCG→GCG); data were combined for gene 1 and gene 2.