Figure 2.

Characterization of various mutant plants. A, T-DNA insertion position in the corresponding gene. Black boxes indicate the coding sequence. White boxes indicate untranslated regions. Triangles indicate the position of T-DNA. B, The corresponding full-length complementary DNAs (cDNAs) were amplified by reverse transcription-PCR to confirm the mutants. C, Ten-day-old seedlings germinated directly on Fe-deficient (–Fe) medium. D, Phenotypes of amiR-bhlh34/104 and bhlh34bhlh104 plants in soils. Four-week-old plants grown in normal soil are shown. WT, Wild type.