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. 2016 Feb 26;170(4):2478–2493. doi: 10.1104/pp.15.01827

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Figure 7. — bHLH34 and bHLH104 activate the promoter of bHLH101. A, Schematic representation of the constructs used for transient expression assays. The reporter construct consists of a bHLH101 promoter, a nuclear localization sequence (NLS) fused with the GFP coding sequence, and a poly(A) terminator. Effector constructs express Myc-bHLH34, Myc-bHLH104, and Myc-MYC2 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. B, bHLH34 and bHLH104 activate the promoter of bHLH101 in transient expression assays. The results are one representative of three biological repeats. C, GFP transcript abundance. In the transient assays, Pro_35S:Myc-GUS was expressed as a control. GFP transcript abundance was normalized to GUS transcript. The value with the empty vector as an effector was set to 1. The results are means ± sd of three technical repeats from one of three biological repeats. Significant differences from the empty vector are indicated by asterisks (P < 0.05).