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Interaction between bHLH34 and bHLH104. A, Yeast two-hybrid assays. Representative growth status of yeast cells is shown on synthetic dextrose medium agar plates without Leu/Trp/His/adenine. B, BiFC assays. N. benthamiana leaves were infiltrated with different combinations of the constructs. C, Coimmunoprecipitation (IP) assays. Total protein was immunoprecipitated using Flag antibody, and coimmunoprecipitated protein was then detected using Myc antibody.
