Figure 5.

RNA-seq analysis of wild-type and wp1s seedlings. mRNA enriched from total RNA isolated from 10-d-old seedlings of wild type and wp1s using oligo-(dT) was fragmented and reverse-transcribed using random hexamer primers. The library was then constructed and sequenced using an Illumina HiSEquation 2000. A, Frequencies of detected genes sorted according to their expression levels. B, Read numbers of wild-type and wp1s sequences. C, Volcano plot showing the overall alterations in gene expression in wild type and wp1s.