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. 2016 Feb 16;170(4):2040–2051. doi: 10.1104/pp.15.01945

Figure 1.

Figure 1.

Complementation of the hema1 knockout mutant by A1 and A1ΔHBD. A, Schematic overview of the GluTR1 domains (Moser et al., 2001). The transit peptide (predicted by TargetP 1.1; Emanuelsson et al., 2007) is followed by the postulated HBD (Vothknecht et al., 1998; Goslings et al., 2004). Binding of FLU (FBD) is mediated by the C terminus of GluTR1 (Goslings et al., 2004). The truncated GluTR1 expressed in the A1ΔHBD lines misses the HBD. B, The A1ΔHBD lines expressing GluTR1ΔHBD and the control line A1 in comparison with the wild type (Col-0) and hema1. C, Pigment content based on fresh weight (FW) and Chl a/b ratio. D, Heme content related to fresh weight. E, Relative quantification of HEMA1 (black) and HEMA2 (gray) mRNA levels as compared with ACT2. F, Immunoblot analysis of total protein extracts. All analyzed plants were cultivated for 28 d on Suc-containing media under SD conditions (10 h light/14 h dark). Data are given as means ± sd (n = 4).