Figure 5.

Pigment and protein analyses are in accord with qE as the major form of NPQ during N deprivation. A, Pigment accumulation (n = 3 ± sd) relative to Chl a in the presence and absence of N in the growth medium following 3 d of N deprivation (black and white bars, respectively). A, Antheraxanthin; L, lutein; V, violaxanthin; Z, zeaxanthin; β, β-carotene. B, LHCSR3 and LHCBM1 accumulation following 3 d of N deprivation assayed by immunoblots under nutrient-replete (N+) and N-deplete (N−) growth conditions for the wild type (WT) and NPQ mutant strains. The samples representing 100% nutrient-replete growth conditions and N-deplete growth conditions were 0.125 and 0.15 μg of Chl, respectively. Different amounts of wild-type membranes were analyzed, as indicated.