Regulation of HS-responsive genes in pollen at tetrad stage. A to C, Relative expression (2–ΔΔCt) is shown for HsfA1a and HsfA2 (A), genes depending on HsfA1a and HsfA2 for higher expression (B), and genes requiring HsfA1a but not HsfA2 (C) in tetrads from wild-type, A2AS-3, A2AS-15, and A1CS plants. Transcript abundance of HsfA2 normalized to wild-type tetrads. Asterisks indicate statistically significant differences (P < 0.05) based on pairwise t test between the wild type and A2AS-3, A2AS-15, or A1CS.