. 2016 Feb 25;170(4):2461–2477. doi: 10.1104/pp.15.01913

Table I. Metabolites affected by HsfA2 suppression in control or heat-stressed anthers Letters indicate statistically significant differences (P < 0.05) as indicated by pairwise t test comparisons. The ±sd for each treatment is indicated (n = 5). WT, wild type.

Class	Name	Retention Time (min)	Match Factor	∆ Retention Index	WT Control		WT Heat Stress		A2AS Control		A2AS Heat Stress
Alcohol	1,2,3-Butantriol	13.90	655	56.68	3,780^b	±385	5,016^a	±823	2,778^c	±582	3,147^bc	±455
Amino acid	γ- aminobutyric acid	7.31	937	18.12	60,016^b	±9,755	82,222^a	±8,462	49,377^b	±8,036	60,699^b	±12,546
	l-Ser	5.75	952	−16.45	40,524^ab	±10,216	46,117^a	±15,824	25,392^b	±7,614	32,806^ab	±8,199
	l-Phe	8.60	727	82.53	3,475^b	±480	5,004^a	±629	2,457^d	±619	3,245^bc	±325
	l-Tyr	10.67	769	103.91	4,921^c	±942	9,788^a	±850	3,573^d	±790	6,952^b	±1,332
	l-Val	4.33	874	−5.60	5,955^c	±583	9,496^a	±761	4,935^d	±572	7,270^b	±1,028
Organic acid	Glyceric acid-3-P	9.29	726	12.57	2,418^b	±310	4,324^a	±773	2,403^b	±343	2,968^b	±427
	Citric acid	9.60	910	52.72	20,623^b	±13,040	14,156^b	±3,034	32,393^c	±13,562	49,168^a	±7,144
	2,3-Dihydroxybutanedioic acid	7.60	905	−15.20	15,976^b	±1,146	19,963^a	±3,868	13,150^b	±1,753	16,491^ab	±3,481
	Citramalic acid	6.87	700	19.31	1,770^a	±124	2,086^a	±235	1,320^b	±232	1,814^a	±315
	Pentonic acid	8.51	857	−45.11	8,626^b	±334	11,046^a	±1,928	7,573^b	±902	9,087^b	±982
	Galactonic acid	10.15	905	−32.10	42,050^b	±5,056	50,100^a	±6,956	31,707^c	±3,658	38,715^bc	±6,816
	Gluconic acid	10.30	872	−13.21	8,590^b	±988	10,692^a	±2,178	7,224^b	±797	8,197^b	±849
	Putrescine	8.38	904	−52.92	34,036^b	±6,882	43,149^a	±6,009	23,797^c	±6,205	26,103^c	±6,307
	Succinic acid	5.88	884	32.02	28,793^b	±1,925	37,527^a	±7,699	23,408^b	±3,484	27,117^b	±4,641
Phosphate	Phosphoric acid	5.35	934	27.46	63,899^a	±2,204	64,838^a	±2,333	59,068^b	±2,267	63,123^a	±3,625
Sugar	Fru	9.48	944	−21.02	84,662^a	±2,847	77,245^b	±2,350	81,132^a	±2,867	69,483^c	±4,430
	Fru-6-P	12.44	530	49.84	898^b	±70	1,056^a	±123	722^c	±105	827^bc	±151
	Maltose	13.97	625	−107.40	7,107^b	±733	9,285^a	±2,248	5,810^b	±1,049	5751^b	±964
	Melibiose	15.97	740	−12.76	4,343^bc	±452	5,559^a	±432	3,824^c	±372	4591^b	±567
	Pentitol	7.84	697	−80.70	721^bc	±120	1,266^a	±266	652^c	±150	941^b	±169
	Rhamnose	8.35	642	−33.39	2,792^c	±383	9,015^a	±1,943	2,425^cd	±473	6,093^b	±1,682
	Xyl	8.07	862	−8.97	10,435^a	±2,804	10,080^a	±1,480	10,088^a	±2,034	7,004^b	±860
	Glc	9.57	941	−21.30	80,963^a	±7,976	64,851^b	±7,848	90,324^a	±8,774	81,349^a	±6,638
	Glc-6-P	12.54	882	53.71	8,528^ab	±781	9403^a	±746	7,038^c	±849	7,618^bc	±1,403
	β-d-Methylfructofuranoside	9.04	843	10.31	10,992^ab	±1,823	11,825^a	±2,800	5,762^c	±3,038	8,245^abc	±2,928
	Fuc	8.46	578	−26.55	7,332^b	±501	9,795^a	±1,495	6,278^b	±824	6,890^b	±708