NAC1 is involved in de novo root organogenesis. A to C, Adventitious rooting from leaf explants of Col-0 (A), NAC1pro:NAC1-SRDX (B), and 35Spro:NAC1-SRDX (C) on B5 medium. Note that both transgenic lines showed defective rooting at 10 DAC. D, Rooting rate analyses of leaf explants from Col-0, NAC1pro:NAC1-SRDX, and 35Spro:NAC1-SRDX. Bars show sd from three biological repeats; n = 30 in each repeat. E, Adventitious rooting from stem explants of Col-0 (left) and 35Spro:NAC1-SRDX (right) cultured on B5 medium, showing rooting defect in 35Spro:NAC1-SRDX. Bars = 1 mm in A to C.