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. 2016 Feb 5;170(4):2136–2145. doi: 10.1104/pp.15.01733

Figure 4.

Figure 4.

NAC1 acts independently of auxin-mediated cell fate transition. A and B, GUS staining of DR5pro:GUS leaf explant cultured on B5 medium at time 0 (A) and 2 DAC (B). C, GUS staining of DR5pro:GUS leaf explant at 2 DAC on medium containing NPA. Note that the GUS signal did not accumulate in wounded region. D, GUS staining of NAC1pro:NAC1-GUS in leaf explant at 2 DAC cultured on B5 medium containing NPA. E and F, qRT-PCR analysis of GH3.2 (E) and NAC1 (F) transcript levels in leaf explants cultured on B5 medium without or with NAA. Bars show sd from three PCR experiments. Results were confirmed in three biological repeats. Data from one repeat are shown. G to J, GUS staining of WOX11pro:GUS (G and H) and WOX11pro:GUS/35Spro:NAC1-SRDX (I and J) leaf explants cultured on B5 medium at 2 DAC. K to N, GUS staining of WOX5pro:GUS (K and L) and WOX5pro:GUS/35Spro:NAC1-SRDX (M and N) leaf explant cultured on B5 medium at 6 DAC. RAM, root apical meristem. H, J, L, and N, Enlargements of boxed regions in G, I, K, and M, respectively. Bars = 500 μm in A to D, G, I, K, and M and 100 μm in H, J, L, and N.