Figure 5.

NAC1 promotes expression of CEP genes. A, RNA-seq analysis using 2-DAC leaf explants from pER8:3×FLAG-NAC1. Leaf explants were cultured on B5 medium containing β-estradiol/DMSO or DMSO only (control). B, Analysis of upregulated genes in RNA-seq data as indicated in A. Also see Supplemental Table S2. C and D, qRT-PCR analysis of CEP1 (C) and CEP2 (D) transcript levels in leaf explants of pER8:3×FLAG-NAC1 cultured on medium without (−) or with (+) 10 μm β-estradiol at 2 DAC. E and F, qRT-PCR analysis of CEP1 (E) and CEP2 (F) transcript levels in leaf explants of 35S_pro:NAC1-SRDX at 4 DAC. Bars in C to F show sd from three PCR experiments. Results were confirmed in two biological repeats. Data from one biological repeat are shown.