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. 2016 Feb 25;170(4):1999–2023. doi: 10.1104/pp.15.01777

Figure 2.

Figure 2.

Immunoblot analysis of Triton extracts from microsomal membranes of transgenic P. pastoris cells. Triton extracts (20–25 μg proteins) were either analyzed under denaturing/reducing (D/R) conditions (1-D SDS-PAGE) or under nonreducing/nondenaturing (ND/NR) conditions (gels contain 0.05% cholate instead of SDS and no DTT) without boiling, as described by Zeng et al. (2010). A, Triton extracts from yeast cells coexpressing TaGT43-4, TaGT47-13, TaGT75-3, and TaGT75-4. B, Triton extracts from yeast cells coexpressing only TaGT43-4 and TaGT47-13. C, Triton extracts from yeast cells expressing TaGT43-4 or TaGT47-13 individually that were mixed (1:1 ratio) before analysis. Polyacrylamide gels were analyzed by immunoblot using anti-TaGT43-4, anti-TaGT47-13, and anti-PsRGP1 antibodies. Molecular weight markers (kD) are indicated.