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. 2016 Feb 25;170(4):1999–2023. doi: 10.1104/pp.15.01777

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Figure 4. — Trafficking of YFP-TaGT43-4 and TaGLP-YFP when transiently expressed in combination with untagged TaGT47-13, TaGT43-4, and/or TaGLP in epidermal cells of tobacco leaves. Coinfiltrated constructs are indicated by the “+” at the left of the figure. ER marker (GFP-HDEL) and trans-Golgi marker (ST-GFP) were used to show the colocalization with YFP-tagged proteins. GFP and YFP fluorescence are shown in green and red, respectively, and their colocalization appears in yellow. Note that YFP-TaGT43-4 export from the ER relies on the presence of TaGT47-13 (B), while the presence of TaGLP does not affect its localization (A). TaGLP-YFP export from the ER requires the presence of both TaGT43-4 and TaGT47-13 (E), while the presence of TaGT43-4 alone does not affect its localization (C). The insets in merge pictures in B and E show the overlap between YFP and ST-GFP. Bars = 10 μm.