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. 2016 Feb 25;170(4):1999–2023. doi: 10.1104/pp.15.01777

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Figure 8. — Self-assembly of TaGT43-4, TaGT75-3, TaGT75-4, TaGT47-13, and TaGLP was investigated through BiFC (split-YFP). Both TaGT43-4 and TaGLP can form homodimers that localize to the ER (A and D, respectively). TaGT75-3 and TaGT75-4 can form homodimers mostly localize to the Golgi (G and I, respectively) and can also form heterodimers (H). TaGT47-13 cannot self-assemble (F). Interaction of TaGT43-4 homodimers with TaGT47-13 results in the shift of the fluorescence to the trans-Golgi (B), while TaGLP homodimers are retained in the ER until interaction with both TaGT43-4 and TaGT47-13 (E). ER marker (GFP-HDEL) and trans-Golgi marker (ST-GFP) were included to show the localization of the assembled YFP. GFP and YFP fluorescence are shown in green and red, respectively, and their colocalization (merge) appears in yellow. Bars = 10 μm.