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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: J Bone Miner Res. 2015 Jul 14;30(11):1959–1968. doi: 10.1002/jbmr.2556

Table 1.

14CO2 Production From 14C-Labeled Glucose in Differentiated MC3T3-E1 Cells

Vehicle PTH
14C position C3,4 C6 C1 C1-C6 C3,4 C6 C1 C1-C6
14CO2 radioactivity (dpm) 39475.6 ± 10275.9 7543.9 ± 1157.7 11281.3 ± 1599.5 6525.5 ± 1599.5 21952.8 ± 5428.4 4755.7 ± 398.6 16575.8 ± 3529.9 11820.1 ± 3529.9
Total radioactivity uptake (dpm) 214263.6 ± 46184.9 212074.4 ± 28164.9 239483.7 ± 27663.2 NA 308221.2 ± 34586.9 304272.8 ± 29502.5 339508.4 ± 25983.7 NA
14CO2 production rate (%) 19.3 ± 7.7 3.6 ± 0.7 4.7 ± 0.6 1.1 ± 0.6 7.3 ± 2.6 1.6 ± 0.2 4.9 ± 1.2 3.4 ± 1.2
14CO2 production index (%/106 cells) 43.9 ± 17.6 8.2 ± 1.7 10.8 ± 1.4 2.5 ± 1.4 17.2 ± 6.2 3.6 ± 0.5 11.6 ± 3.1 7.9 ± 2.9

Values are mean ± SD; n = 4 or 5.

Radioactivity uptake = 444,000 dpm × % glucose consumption; 14CO2 production rate (%) = 14CO2 radioactivity/total radioactivity uptake; 14CO2 production index (%/106 cells) = 14CO2 production rate/cell number.