Figure 1.

Colocalization of green fluorescent protein (GFP)⁺ and glial fibrillary acidic protein (GFAP)⁺ astrocytes. Sections of amyloid precursor protein (APP)-Swedish-Dutch-Iowa (SweDI) × GFP-GFAP mice were immunohistochemically stained for GFAP (B–E). In a cluster of reactive astroglia, many cells were double positive for both GFP (green, A) and GFAP (red, indicated by an arrow, A–C). However, some astrocytes were positive for either GFP or GFAP (A–C). Confocal microscopy of brain sections clearly shows double positive astrocytes (indicated by a thick arrow D,E) adjacent to an astrocyte being merely positive for GFAP (indicated by thin arrows, D). Scale bar = 35 μm (A–C), 6 μm (D,E).