Figure 3.

CKS1 expression determines body size. (A) phenotypes after dsCKS1 injection at different larval stages (fifth 12 h, sixth 6 h, sixth 24 h and sixth 48 h, 500 ng/larva, 24 h interval for 3 times injection), dsGFP injection at different larval stages with the same condition as the control group; (B) qRT-PCR to detect the effects of RNAi in the midgut, 24 h post the first dsRNA injection. The asterisks indicate significant differences from the control group (P < 0.05) by using the student's t test. (C) calculation of average pupae weight in (A); (D) calculation of average mortality rate in (A). The average was from 3 repeats (20 larvae × 3).