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Examination of midgut cell proliferation and PCD from 4th instar to 6th instar based on immunohistochemistry analysis. Panels a1 to h1, DAPI staining showing the nuclei of midgut cells; a2 to h2, magnification of a1 to h1; a3 to h3, phospho-histone 3 detection using the antibody and goat anti-mouse IgG Alexa-Fluor 488 (green) showing cell proliferation; a4 to h4, TUNEL staining showing the midgut PCD. Bar indicates 50 μm.
