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. 2015 Jul 22;14(19):3045–3057. doi: 10.1080/15384101.2015.1053664

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Figure 5. — Midgut cell proliferation occurs in the larval regenerative cells. Panel 1, HE staining showing the midgut cells in fourth instar feeding larvae, cc: columnar cells, gc: goblet cells, rc: regenerative cells; panel 2, phospho-histone 3 antibody detection showing the proliferating cells; panel 3, DAPI staining showing the cell nucleus; panel 4, merged image of panels 2 and 3; panel 5, HE staining showing the midgut of sixth instar 96 h larvae, LM: larval midgut, IM: imaginal midgut; panel 6, phospho-histone 3 antibody detection; panel 7, DAPI staining; panel 8, merged image of panels 6 and 7. Bar indicates 20 μm.