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. 2015 Jul 22;14(19):3045–3057. doi: 10.1080/15384101.2015.1053664

Figure 6.

Figure 6.

CKS1 knockdown represses cell proliferation and gene expression in the larval midgut. dsCKS1 was injected to the fifth instar 12 h larvae twice, 48 h interval, 500 ng/larva. dsGFP injection was the non-specific RNAi control. (A) Immunohistochemistry showing the repress of midgut cell proliferation by CKS1 knowckdown. Panel 1, efficacy of CKS1 knockdown examined at the sixth instar 24 h larvae; panel 2, phenotypes related to panel 1; panels 3 to 5, detection of cell proliferation after dsGFP injection by the antibody against phospho-histone 3 and goat anti-mouse IgG Alexa-Fluor 488 (green) second antibody; panels 6 to 8, detection of cell proliferation by anti-phospho-histone 3 primary antibody after dsCKS1 injection. Bar indicates 50 μm. The white arrows pointed to the amplification of the pictures. (B) HE-stained midguts at 6th 96 h after dsGFP or dsCKS1 was injected. LM: larval midgut, LU: lumen of midgut, IM: imaginal midgut. The scale bar is 50 μm. (C) qRT-PCR showing the gene expression after CKS1 knockdown in larval midgut. Asterisks indicate significant differences from the control group (P < 0.05) by using the student's t test based 3 independent repeats.