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qRT-PCR showing the regulation of CKS1 by insulin via InR. (A) Knockdown of InR, (B) knockdown of PI3K, (C) knockdown of AKT, (D) knockdown of FOXO. 1*PBS is used as the solvent control for insulin, dsGFP as the non-specific dsRNA control for dsCKS1. These experiments were repeated 3 times and statistically analyzed. The asterisks indicate significant differences from the control group (P < 0.05) by using the student's t test.
