Figure 1.

Pfn1 overexpression causes p27 accumulation in MDA-231 cells through inhibiting its protein turnover. (A) P27 immunoblot of total cell extracts prepared from sub-confluent cultures of GFP- and GFP-Pfn1-expressing MDA-231 cells following treatment with 10 μM of either MG-132 (a proteasome inhibitor) or DMSO (vehicle control) for 10 hours. Tubulin blot serves as a loading control. (B) P27 and skp2 immunoblots of cytosolic and nuclear extracts prepared from GFP- and GFP-Pfn1-expressors (tubulin and histone blots served as loading control for cytosolic and nuclear fractions, respectively). These data are representative of 2–3 independent experiments.