Figure 2.

Pfn1 overexpression has site-specific effects on p27 phosphorylation in MDA-231 cells. (A) Total lysates prepared from sub-confluent cultures of GFP- and GFP-Pfn1-expressing MDA-231 cells were separated by 2D-gel electrophoresis and immunoblotted with p27 antibody. Higher amount of total protein was loaded for the GFP group to normalize the total p27 level between 2 groups. (B) Total cell extracts of GFP- and GFP-Pfn1 expressors were separated on an SDS-PAGE and immunoblotted with the indicated antibodies (tubulin blot serves as a loading control). These data are representative of 2–3 independent experiments.