Figure 4.

Spermatogenesis in Chfr knockout male mice with small testes are delayed at 3 and 4 weeks. Testis sections of 3 and 4-week-old male mice were stained with periodic acid schiff (PAS)-hematoxylin. Typical pictures of normal testes from WT male mice (a-d for 4 weeks and k-m for 3 weeks) and small testes from Chfr knockout male mice (e-h for 4 weeks and n-p for 3 weeks) are shown. Scale bar, 50 µM. Asterisk: large vacuoles. (i-j) For 4-week-old male mice, seminiferous tubules with multiple layers of germs cells (i) and with large vacuoles (j) are summarized. Mean and standard deviation are shown.