Figure 5.

Apoptosis occur in germ cells from Chfr knockout male mice. (a-b) Apoptosis was detected in testis sections of 3 and 4-week-old male mice. Apoptotic cells are marked by arrow heads. Seminiferous tubules with apoptotic cells are summarized. Scale bar, 50 µM. (c) Levels of ATM, phosphorylated ATM at serine 1981, Chk2, and phosphorylated Chk2 are shown in WT and Chfr knockout MEFs with or without ionizing radiation (IR). (d) Levels of ATM, phosphorylated ATM at serine 1981, Chk2, and phosphorylated Chk2 are shown in 3-week-old WT and Chfr knockout testes. N, normal size testes. S, small testes. Quantification was performed. Mean and standard deviation are shown.